The c-ring stoichiometry of ATP synthase is adapted to cell physiological requirements of alkaliphilic Bacillus pseudofirmus OF4
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منابع مشابه
Roles of AtpI and two YidC-type proteins from alkaliphilic Bacillus pseudofirmus OF4 in ATP synthase assembly and nonfermentative growth.
AtpI, a membrane protein encoded by many bacterial atp operons, is reported to be necessary for c-ring oligomer formation during assembly of some ATP synthase complexes. We investigated chaperone functions of AtpI and compared them to those of AtpZ, a protein encoded by a gene upstream of atpI that has a role in magnesium acquisition at near-neutral pH, and of SpoIIIJ and YqjG, two YidC/OxaI/Al...
متن کاملPurification and Reconstitution of the FIFO-ATP Synthase from Alkaliphilic Bacillus firmus OF4 EVIDENCE THAT THE ENZYME TRANSLOCATES
The FIFO-ATP synthase from the alkaliphilic Bacillus firmus OF4 was purified in a reconstitutively active form, in good yield and with a high specific ATPase activity when appropriately activated. The purification procedure involved octyl glucoside extraction of washed membrane vesicles in the presence of 20% glycerol and asolectin followed by ammonium sulfate fractionation and sucrose density ...
متن کاملCrystallization and X-ray diffraction of an ectoine hydroxylase from Bacillus pseudofirmus OF4.
OBJECTIVE To study crystallization and X-Ray diffraction of ectoine hydroxylase from Bacillus pseudofirmus OF4. METHODS We cloned the gene BpectD from B. pseudofirmus OF4 with a His6 tag and overexpressed it in E. coli BL21 (DE3). Then, we purified protein BpEctD by Ni2 -chelating affinity and size-exclusion chromatography. After that, crystals were grown by the sitting-drop vapour-diffusion ...
متن کاملCharacterization of endogenous pyridoxal 5'-phosphate-dependent alanine racemase from Bacillus pseudofirmus OF4.
An open reading frame of 1100 bp in the partially sequenced genome sequence of alkaliphilic Bacillus pseudofirmus OF4 was identified as a putative alanine racemase gene (dadX(OF4)), which was cloned and expressed in Escherichia coli BL21 (DE3). The encoded protein DadX(OF4) was purified to homogeneity by His(6)-tag affinity column, gel filtration and ion-exchange chromatography. The amino acid ...
متن کاملRole of the nhaC-encoded Na+/H+ antiporter of alkaliphilic Bacillus firmus OF4.
Application of protoplast transformation and single- and double-crossover mutagenesis protocols to alkaliphilic Bacillus firmus OF4811M (an auxotrophic strain of B. firmus OF4) facilitated the extension of the sequence of the previously cloned nhaC gene, which encodes an Na+/H+ antiporter, and the surrounding region. The nhaC gene is part of a likely 2-gene operon encompassing nhaC and a small ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2013
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1303333110